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Test Code KITBM KIT Asp816Val Mutation Analysis, Qualitative PCR, Bone Marrow

Reporting Name

KIT Asp816Val Mutation Analysis, BM

Useful For

Diagnosing systemic mastocytosis

Method Name

Allele-Specific Oligonucleotide Polymerase Chain Reaction (PCR)
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Bone Marrow

Specimen Required

The following information is required:

1. Pertinent clinical history

2. Clinical or morphologic suspicion

3. Date of collection

4. Specimen source


Specimen must arrive within 168 hours of collection.


Container/Tube: Bone marrow

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD solution B)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube.

3. Label specimen as bone marrow.


1. Hematopathology Patient Information Sheet (Supply T676) in Special Instructions

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request Form (T726) with the specimen


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Bone Marrow Ambient (preferred) 7 days
  Refrigerated  7 days

Reject Due To


Mild OK; Gross reject







Reference Values

An interpretive report will be provided indicating the mutation status as positive or negative.

Day(s) and Time(s) Performed

Monday through Friday

CPT Code Information

81402-KIT (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) (eg, mastocytosis), common variants (eg, D816V, D816Y, D816F)

LOINC Code Information

Result ID Reporting Name LOINC Code
34852 Final Diagnosis: 34574-4

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.